human peripheral blood cd14 + monocytes cryopreserved zenbio ser-cd14-f  (ZenBio)

Journal: Cell Death & Disease

Article Title: Dexamethasone drives macrophage repolarization linked to increased triple-negative breast cancer aggressiveness

doi: 10.1038/s41419-025-08363-9

Figure Lengend Snippet: A Schematic of DEX treatment during macrophage differentiation (day 0–6). B mRNA expression of the M1 markers CCR7 , CD80 , PTGS2 , and IL1B ( n = 3 [ CCR7 ], 4), and C ) M2 markers CD163 and MRC1 ( n = 3). D Schematic of DEX treatment during macrophage M1 polarization (day 6–8). E mRNA expression of M1 markers ( n = 3 [ PTGS2, IL1B ], 5 [ CCR7, CD80 ]), and F ) M2 markers ( n = 3, 4 [M2 0, M1 0]). H Schematic of DEX treatment of M1 polarized macrophages (days 8–10). I mRNA expression of M1 markers ( n = 3) and J M2 markers ( n = 3). M2 macrophages were not DEX-treated, and comparisons were to M1 polarized macrophages at 0 nM DEX in ( A )–( J ). K mRNA expression of the M2 markers CD163 and MRC1 , and NR3C1 (GR) in primary human peripheral blood monocyte-derived macrophages (hMDMs) after 2 days of vehicle or 100 nM DEX treatment. All mRNA expression was relative to untreated THP-1 cells and normalized to the housekeeping gene HPRT1 in B , C , E , F , I , J , and relative to untreated CD14 + hPBM cells and normalized to HPRT1 in K. Bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparisons correction in B , C , E , F , I , J , and using Student’s unpaired t -test in ( K ).

Article Snippet: In addition, primary CD14 + human peripheral blood monocytes (hPBMs) were purchased from PromoCell (#C-14110) as a single-donor cell pellet stored in RNA later ® .

Techniques: Expressing, Derivative Assay

Journal: bioRxiv

Article Title: Responders vs. non-responders to mesenchymal stromal cells in knee osteoarthritis patients: mechanistic correlates of donor cell attributes and patient features

doi: 10.1101/2025.07.17.665028

Figure Lengend Snippet: A) Higher levels of resistin significantly positively correlated to delta values in KOOS Sports/Rec scores at 24 months. Higher levels of TIMP1 and CCL2 significantly correlated inversely to the percentage change and/or delta in KOOS Sports/Rec scores at 24 months relative to baseline. B) Higher levels of HGF and VEGFA in synovial fluid significantly correlated inversely to delta values and/or percentage change in KOOS Pain scores at 12 months relative to baseline. C) Higher levels of sCD14 and CCL2 significantly correlate inversely to the percentage change and/or delta in KOOS Symptom and Sports/Rec scores at 3 months relative to baseline. Delta and percent change in KOOS values were made relative to baseline and calculated such that positive values represent improvement. N=8-9 patients. Spearman’s correlation. Sports/Rec: function in sport and recreation; MO: month; KOOS: knee injury and osteoarthritis outcome score; TIMP1: tissue inhibitor of metalloproteinases 1; CCL2: chemokine C-C motif ligand 2; HGF: hepatocyte growth factor; sCD14: soluble CD14; VEGFA: vascular endothelial growth factor A.

Article Snippet: Indirect co-culture of unmatched human CD14 + peripheral blood-derived monocytes (STEMCELL Technologies, Vancouver, Canada) with biobanked MSC(M) was performed as previously described [ ].

Techniques: